Journal: Frontiers in Neural Circuits

Article Title: Reconstructing neural circuits using multiresolution correlated light and electron microscopy

doi: 10.3389/fncir.2022.753496

Figure Lengend Snippet: Example confocal imaging of fixed retina (top) and visual thalamus (bottom) to map features for mrCLEM. (A) Imaging of the ganglion-cell-side surface of a mouse retina (transgenic labeling green) with reflected 405 laser line (blue) and autofluorescence from the 633 laser line (red). Note clear cell bodies and vasculature. (B) Imaging of the ganglion cell layer in mouse retina (transgenic labeling green) with Sulforhodamine (red), and DAPI (blue). (C) Same tissue as (B) imaged 50 μm deeper in the inner nuclear layer. (D,E) Six-channel tissue mapping of a vibratome section of mouse dorsal lateral geniculate nucleus. (D) Retinal ganglion cell axons from the contralateral eye labeled with CtB-488 (green), and the ipsilateral eye labeled with CtB-633 (red). Fiber tracks (blue) are imaged using reflected 633 laser line. (E) Same image stack as (D) , showing DAPI (blue), FluoroMyelin (red), and 546 reflected light (green). White box indicates the field of view for (F) . (F) Single plane from a high-resolution image stack acquired at the white box in E. High-frequency matching features (FluoroMyelin = cyan, retinal ganglion cell synaptic boutons = red and green) are shown in the context of medium-resolution features (DAPI stained nuclei = magenta) and large-scale features (fiber tract = white).

Article Snippet: Sections were cut using a Compresstome vibratome (Precisionary VF-200-0Z).

Techniques: Imaging, Transgenic Assay, Labeling, Staining